High-dimensional fluorescence cytometry

In late 2016 we launched our high-dimensional fluorescence cytometry system, the 'LSR-X'. With help from Becton Dickinson (BD), we equipped our world-first 10-laser BD LSR-II special order research product (SORP) platform with a newly developed electronics system, increasing the number of detectible signals to 30 parameters (27 colours, 3 scatter). 

We are currently running panels of 26 colours, using a range of traditional dyes in addition to novel polymer dyes developed by Sirigen. 

In 2017, we published a methods paper detailing our work the development of high-dimensional panels. Ashhurst, T, Smith, A, King, N.J.C. High dimensional fluorescence cytometry. Current Protocols in Immunology, 119, 5.8.1 – 5.8.38. doi: 10.1002/cpim.37.

'LSR-X' (10-lasers, 30-parameters)

LSR-X.png

Custom BD instrument - experimental analyser
Overview
: Experimental 10-laser 29-parameter analyser
Lasers: 355nm, 405nm, 445nm, 488nm, 514nm, 532nm, 561nm, 594nm, 635nm, 786nm
30 parameters: 27 colours, 3 scatter
Speed: sample acquisition of 10,000 events/sec
Capabilities: Samples from 5 mL tubes. Able to attach a high-throughput sampler (96-well plate reader). 

Full instrument configurations are available for registered users on PPMS here. Alternatively, they can be found on the CI wiki. Usage prices can be found here.

 

To request training on the LSR-X, use the link below. For new users, you can find out about getting started with Sydney Cytometry here.

For more information about data analysis approached for high-dimensional data, use this link. For more information on the LSR-X platform, or high-dimensional cytometry, send an email to info@sydneycytometry.org.au.