High-dimensional fluorescence cytometry
In late 2016 we launched our high-dimensional fluorescence cytometry system, the 'LSR-X'. With help from Becton Dickinson (BD), we equipped our world-first 10-laser BD LSR-II special order research product (SORP) platform with a newly developed electronics system, increasing the number of detectible signals to 30 parameters (27 colours, 3 scatter).
We are currently running panels of 26 colours, using a range of traditional dyes in addition to novel polymer dyes developed by Sirigen.
In 2017, we published a methods paper detailing our work the development of high-dimensional panels. Ashhurst, T, Smith, A, King, N.J.C. High dimensional fluorescence cytometry. Current Protocols in Immunology, 119, 5.8.1 – 5.8.38. doi: 10.1002/cpim.37.
'LSR-X' (10-lasers, 30-parameters)
Custom BD instrument - experimental analyser
Overview: Experimental 10-laser 29-parameter analyser
Lasers: 355nm, 405nm, 445nm, 488nm, 514nm, 532nm, 561nm, 594nm, 635nm, 786nm
30 parameters: 27 colours, 3 scatter
Speed: sample acquisition of 10,000 events/sec
Capabilities: Samples from 5 mL tubes. Able to attach a high-throughput sampler (96-well plate reader).
To request training on the LSR-X, use the link below. For new users, you can find out about getting started with Sydney Cytometry here.