Flow cytometry cell sorters

Full instrument configurations are available for registered users on PPMS here. Alternatively, they can be found on the CI wiki. Sorting prices can be found here. Sorter availability can be checked here. Availability should considered as a guide only, as an unbooked period of time does not guarantee instrument availablity.

Sorting requests can only be made after biosafety approval (see this link).

 

Centenary Institute (Level 1)

 
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BD InFlux (10-laser)

Overview: Stream in air sorter
Lasers: 355nm, 405nm, 445nm, 488nm, 514nm, 532nm, 561nm, 594nm, 635nm, 786nm
22 colour + 2 scatter
Capabilities: 

  • 24 parameters (up to 22 colours, 2-3 scatter, including polarised FSC)
  • Up to 30,000 events/sec
  • 1 - 8 way sorting
  • Class II biosafety cabinet
  • Cooled sample delivery and collection
  • Tubes (5-15ml), plates (6-384well), slides
  • Index sorting
  • Nozzles 70-200um (85um, 100um, 140um, 200um)
  • Highly-configurable
 

BD FACSAria IIu

Overview: Stream in air sorter
Lasers: 405 nm, 488 nm, 628 mn
Speed
Capabilities

  • 3 lasers - violet, blue, red
  • 11 colours, 2 scatter
  • Up to 20,000 events/sec
  • 4 way sorting
  • Class I biosafety cabinet
  • Tubes, plates, slides
  • Nozzles 70-130um
 
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BD InFlux (3-laser) PC3 sorter

Overview: Stream in air sorter, located in PC3 laboratory
Lasers: 405 nm, 488 nm, 628 mn
Speed
Capabilities

  • 3 lasers - Violet-405, Blue-488, Orange-594
  • 17 parameters (15 colours, 2 scatter)
  • Up to 30,000 events/sec
  • 8-way sorting
  • Class II biosafety cabinet
  • Tubes, plates, slides
  • Nozzles 70-200um
  • Highly-configurable
 

Charles Perkins Centre (Level 5)

 
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BD InFlux (7-laser)

Overview: Stream in air sorter
Lasers: 355nm, 405nm, 488nm, 514nm, 561nm, 640nm, 786nm
22 colour + 2 scatter
Capabilities: 

  • 24 parameters (up to 22 colours, 2-3 scatter, including polarised FSC)
  • Up to 30,000 events/sec
  • 1 - 6 way sorting
  • Class II biosafety cabinet
  • Cooled sample delivery and collection
  • Tubes (5-15ml), plates (6-384well), slides
  • Index sorting
  • Nozzles 70-200um (85um, 100um, 140um, 200um)
  • Highly-configurable
 
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BD FACSJazz

Overview: Stream in air sorter. A simpler and light version of the Influx. The Jazz is a user operated cell sorter operating within the ACF core facility.
Lasers: 
Capabilities:

  • 2 lasers - blue, red (orange)
  • 6 colours, 2 scatter
  • Up to 10,000 events per second
  • 2 way sorting
  • Class II biosafety cabinet
  • Tubes, plates, slides
  • 100um nozzle