Protocols and panel development

Extensive protocols are available for registered users at this link. If you do not have access to a protocol of interest, email us at info@sydneycytometry.org.au.

 

PANEL BUILDING protocols:

Basic multicolour flow cytometry: Current Protocols in Immunology, Unit 5.4

Building high-dimensional panels: Current Protocols in Immunology, Unit 5.8

STAINING PROTOCOLS:

Titration protocols for 50 uL staining volumes, and 100 uL staining volumes

Standard staining protocol (for facility users: https://wiki.centenary.org.au/x/8IDvBw)

Intracellular staining protocol (for facility users: https://wiki.centenary.org.au/x/AYHvBw)

BrdU protocol (TBC)

INSTRUMENT PROTOCOLS:

Cytometer operation protocol (for facility users: https://wiki.centenary.org.au/x/BoHvBw)