Many users of our core facility use FlowJo software (https://www.flowjo.com/) for the analysis of flow cytometry data.

FLOWJO SOFTWARE

Software can be downloaded from the FlowJo website at https://www.flowjo.com/solutions/flowjo/downloads, and can be used as part of a 30-day free trial without a licence.

LEARNING FLOWJO

When starting out with FlowJo, we recommend the following webpage: http://docs.flowjo.com/d2/getting-acquainted/learn-flowjo-now/

There are also a number of tutorials available, we recommend the basic and 8-colour PBMC tutorials (https://www.flowjo.com/solutions/flowjo/downloads/tutorials).

As the number of parameters that we can measure in cytometry increases, so too does the complexity of the data we are able to generate. As such, manual methods of analysis (such as gating) are not always suitable for the analysis of these datasets. Various computational tools can be harnessed to aid in the analysis of these datasets, but the use of these tools needs to be well considered.