Overview: The Xenium platform and assays facilitate detection of in situ gene expression of hundreds to thousands of RNA molecules using detection probe sin formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples with cellular resolution.

Further information on the Xenium system, as well as assay options and protocol documentation can be found on the 10X Genomics website here: 

https://www.10xgenomics.com/support/in-situ-gene-expression

Overview: CosMx spatial molecular imager (SMI) enables high-plex detection of thousands of RNA molecules in formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples with cellular resolution. CosMx additionally allow for imaging of up to 64 proteins (via antibody detection) but on a subsequent slide (i.e. RNA and protein detected on separate sections.

Further information on the CosMx system, as well as assay options and protocol documentation can be found on the NanoString website here:

https://nanostring.com/products/cosmx-spatial-molecular-imager/single-cell-imaging-overview/

Overview: The Visium platform from 10x Genomics combines histology and spatially resolved whole transcriptome gene expression profiling to localize and quantify gene expression in the tissue context. It is based, in part, on the Spatial Transcriptomics methodology (1). Visium was commercialized in 2019 and has been used in groundbreaking papers demonstrating the breadth of its applications, including cancer (3,4), neuroscience (5), immunology (6), and developmental biology (7). Many researchers have adopted the assay, as evidenced by the over 400 peer-reviewed publications and pre-prints utilizing the technology. Visium Spatial Gene Expression is compatible with a variety of tissue types and species.

Assay options can be explored here under 'Visium': https://www.10xgenomics.com/support

Overview: the PhenoImager HT is the fastest whole-slide multispectral imaging system that can be easily integrated into high-throughput workflows to accommodate for scalability. PhenoImager HT running the newly released 2.0 software provides researchers with a unique technology stack combining onboard spectral unmixing, rapid imaging and manageable data outputs. When combined with PhenoCode Signature assays for 6-plex, 7 Color whole slide imaging, HT 2.0 delivers unparalleled performance for spatial signature development.

https://www.akoyabio.com/phenoimager/instruments/phenoimager-ht/

The Lunaphore COMET™ is an automated, high-plex spatial proteomics platform that enables subcellular resolution imaging across large tissue sections (up to 12.5 × 12.5 mm). Leveraging an automated staining and imaging workflow, COMET performs sequential immunofluorescence to image the expression of up to 40 protein targets per experiment. Panel development is rapid and reagent-agnostic, using standard, unconjugated primary antibodies—eliminating the need for custom conjugation. COMET delivers high reproducibility, flexible panel design, and compatibility with diverse sample types, making it ideally suited for spatial biology and protein expression profiling.