Image Cytometry

Image Cytometry - Instruments

  • Amnis ImageStream MKII

    Overview: 5 Laser, 12 parameter flow cytometer that takes an image of each cell, across each channel. Providing both locational and standard flow cytometer scatter information

    Lasers: Violet, Blue, Yellow-Green, Red and IR (for scatter)
    9 colour + 1 scatter + 2 brightfield parameters

    Sample acquisition of up to 5000 events/second

    Capabilities:
    - 3 Magnifications - 20x, 40x, 60x
    - 96-well autosampler, plus standard tube acquisition
    - Dedicated analysis software

  • PerkinElmer Opera Phenix Plus

    Overview: 5 Laser, 4 Camera, Confocal High-Content Bioimaging System.

    Lasers: 375nm, 425nm, 488nm, 561nm, 640nm

    High-throughput imaging of plate-based assays.

    Capabilities:
    - Spinning disk confocal and widefield imaging
    - Wide variety of objectives:
    - Air: 1.25x, 5x, 10x, 20x, 20x High NA, 40x
    - Water: 20x, 40x, 60x
    - Liquid handling for use during image acquisition
    - User friendly software
    - Integrated image analysis software
    - Highly adaptable software for complex imaging
    - Both plate and slide-based imaging possible

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